A single mutation in the 15S rRNA gene confers nonsense suppressor activity and interacts with mRF1 the release factor in yeast mitochondria
We have determined the lycogel nucleotide sequence of the mim3-1 mitochondrial ribosomal suppressor, acting on ochre mitochondrial mutations and one frameshift mutation in Saccharomyces cerevisiae.The 15s rRNA suppressor gene contains a G633 to C transversion.Yeast mitochondrial G633 corresponds to G517 of the E.coli 15S rRNA, which is occupied by